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Gemi
PCR primers / probes design from multiple & degenerate sequences
...Gemi accepts multiple aligned and long DNA and RNA sequences with degenerate nucleotide (non-A/C/G/T bases). Gemi can be used for quantitative, real-time and conventional PCR (qPCR, rt-PCR, etc.), and Sanger sequencing. Gemi can parse large dataset of sequences efficiently. Python source code is available upon request. Milestone: The tool reached about 3000 downloads sine 2012. Article Gemi: PCR primers prediction from multiple alignments. Comparative and functional genomics 2012 PMID: https://www.ncbi.nlm.nih.gov/pubmed/23316117 A great review on designing primer, Gemi, and other tools: Designing degenerate primers: Overview, challenges, and computational methods. ...
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