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ggplot2 is a system written in R for declaratively creating graphics. It is based on The Grammar of Graphics, which focuses on following a layered approach to describe and construct visualizations or graphics in a structured manner. With ggplot2 you simply provide the data, tell ggplot2 how to map variables to aesthetics, what graphical primitives to use, and it will take care of the rest. ggplot2 is over 10 years old and is used by hundreds of thousands of people all over the world for...

This part of the book teaches you how to leverage the plotly R package to create a variety of interactive graphics. There are two main ways to creating a plotly object: either by transforming a ggplot2 object (via ggplotly()) into a plotly object or by directly initializing a plotly object with plot_ly()/plot_geo()/plot_mapbox(). Both approaches have somewhat complementary strengths and weaknesses, so it can pay off to learn both approaches. Moreover, both approaches are an implementation of...

ggpubr is an R package that provides easy-to-use wrapper functions around ggplot2 to create publication-ready visualizations with minimal code. It streamlines plot creation for researchers and analysts, allowing features such as statistical annotation, theme customization, and plot arrangement with fewer lines of code.

nichenetr: the R implementation of the NicheNet method. The goal of NicheNet is to study intercellular communication from a computational perspective. NicheNet uses human or mouse gene expression data of interacting cells as input and combines this with a prior model that integrates existing knowledge on ligand-to-target signaling paths. This allows to predict ligand-receptor interactions that might drive gene expression changes in cells of interest. This model of prior information on...

In this tutorial, we describe a workflow to ensure long-term reproducibility of R-based data analyses. The workflow leverages established tools and practices from software engineering. It combines the benefits of various open-source software tools including R Markdown, Git, Make, and Docker, whose interplay ensures seamless integration of version management, dynamic report generation conforming to various journal styles, and full cross-platform and long-term computational reproducibility....