multipsq
Analysis of pyrograms form multiplex pyrosequencing experiments
... on a single SNP or short sequence stretch, necessitating the consideration of several genomic regions. A promising rapid approach is the simultaneous application of multiple sequencing primers, called multiplex pyrosequencing. These primers generate a fingerprint-pyrogram which is constituted by the sum of all individual pyrograms originating from each primer used. MultiPSQ allows the analysis and classification of multiplex-pyrograms originating from various pyrosequencing primers.