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SeqHunter2 is a stand-alone software with graphical user interface, running on Microsoft windows system. It is programmed with the following functions: 1) collect and manage local sequence databases; 2) run blast search by using a batch of sequences and with abundant output formats; 3) extract sequences by a set of IDs or coordinates; 4) generate sequences alignment; 5) edit sequence and translate nucleotide to proteins; 6) browse genome and search genes; 7) collect resource items and link to the website. The full version collects all available oomycete plant pathogens genome sequences and information of research related resource, while the lite version is flexible for non-oomycete users to collected the data they are interested.