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A CRISPR tool for user-defined protospacer adjacent motif
CRISPR/Cas system undoubtedly holds great potential for genome editing. Target site cleavage by CRISPR technology requires a protospacer adjacent motif (PAM) immediately downstream or upstream of the protospacer element to which the sgRNA binds. However, Cas9 from different types of bacteria or variant recognizes different PAM sequences. To meet the needs of different CRISPR system with specific and efficient sgRNA design, CRISPR-offinder was developed.
Given an input FASTA file of the...
Accurately discover viral integration events and fusion transcripts
...VFS combines soft-clipping information, read-pair analysis, and targeted de novo assembly to discover and annotate viral-human fusion events.
A simple yet effective empirical statistical model is used to evaluate the quality of fusion breakpoints.
Minimal user defined parameters are required.
Source code with user manual and installation guide of VFS is available at sourceforge's "Files" section.
Citation: http://www.ncbi.nlm.nih.gov/pubmed/
A simple tool to design DNA primers for mutagenesis in BGME lab (JHU). Allows users to select or enter background sequence and add current and new mutations. During the design process, users are presented with various updating checks to guide them.
Molecular Graphics and Dynamics. Molecular model builder. Part of the Ascalaph Designer http://sourceforge.net/projects/asc-designer/ project. Windows & Linux.
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Surviving bots is continuous software application in Java that will guide robots to search for energy source around it, tap it and explore ahead for survival and thus evolve into much more advance system.Software is ment to be hardware independent.
BIRBU (BIological Relationship BUilder) is a Java tool for microarray gene expression data analysis. BIRBU identifies biologically significant relationship between genes using microarray data and prior knowledge on relationships between genes.