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    Power Decoder

    The Power Decoder simulator for the evaluation of pooled shRNA screen

    RNA interference (RNAi) screening using pooled, short hairpin (sh)RNA is a powerful, high-throughput tool for determining the biological relevance of genes for a phenotype. Assessing an shRNA pooled screen’s performance is difficult in practice; one can estimate the performance only by using reproducibility as a proxy for power or by employing a large number of validated positive and negative controls. Here, we develop an open-source software tool, the Power Decoder simulator, for generating...
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