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...There are also instructions provided in case you are running these analyses via a computing cluster/Slurm workload manager. You can choose to go through the workflow using either an Rmd script, an html file, or a PDF, or via the homepage link provided. If you have questions or concerns, please don't hesitate to reach out. Thanks!

...The central anchor for the data congregation is the modificationSpecificPeptides.txt or diann-output.pr_matrix.tsv file - additional data is inferred from different other source files from the corresponding folder. Maybe also useful for normal proteomics purposes but this script is heavily optimized for protein neo-termini identification and validation. A graphical interface is available as Yoğurtlu_MANTI (a Perl/Tk script) + execut. application versions for Win1x without the need to have Perl installed locally. For a very detailed explanation of script parameters and the evaluation strategy, please consult the extensive manual PDF...

...Maybe also useful for normal proteomics purposes but this script is heavily optimized for protein neo-termini identification and validation. For a more thorough explanation of script parameters and evaluation strategy, please consult the extensive manual PDF.

The NOFI ranking algorithm has been developed to assign low priority to fragment ions affected by interference. The implementation is an R script that can be customized. A future version will contain a modular version. The outline is as follows: 1) The input contains the list of SWATH fragment ion XICs from the identified and quantified peptides by software tools such as Skyline and OpenSWATH. 2) The first step in NOFI is the computation of the 4 attributes (RTd, FWHMd, IRd and IRrep) used to represent each fragment ion as a vector. 3) Multivariate outlier detection techniques are used to rank all the fragment ions from each peptide. 4) Several figures are generated (a pdf file) to visualize the effect of the Top-N fragment ions over different indicators. 5) The user can choose the number of top fragment ions per peptide, thereby utilizing the optimal subset of high priority Top-N NOFIs for quantification while excluding the impaired fragment ions.

ProPHAnE: Proteomics result Pruning and Homology group Annotation Engine A perl script based workflow for annotation of protein data coming from the 'Mascot' (Matrix Science) and 'Scaffold' (Proteome Software) pipeline. Coming soon: Prophane 2, "a much improved and extended version of Prophane based on HTML5, PHP and MySQL. Most importantly, the pipeline includes now functional predictions on profile Hidden Markov models (HMMs), is fully-automated, and can be controlled via a highly intuitive and user-friendly interface." ...