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Opera (Optimal Paired-End Read Assembler) is a sequence assembly program (http://en.wikipedia.org/wiki/Sequence_assembly). It uses information from paired-end or long reads to optimally order and orient contigs assembled from shotgun-sequencing reads.
An updated version called OPERA-LG has been re-engineered with features for the assembly of large and complex genomes.
Song Gao, Denis Bertrand, Burton K. H. Chia and Niranjan Nagarajan. OPERA-LG: efficient and exact scaffolding of large, repeat-rich eukaryotic genomes with performance guarantees. Genome Biology, May 2016, doi: 10.1186/s13059-016-0951-y.
Song Gao, Wing-Kin Sung, Niranjan Nagarajan. Opera: reconstructing optimal genomic scaffolds with high-throughput paired-end sequences. Journal of Computational Biology, Sept. 2011, doi:10.1089/cmb.2011.0170.
Features
- Assembly with performance guarantees
- Assembly of large and complex genomes
- Supports third-generation sequencing data (PacBio, Nanopore)
- Assembly of repeat sequences
- Improved memory and runtime efficiency
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User Reviews
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Operasf is excellent! Thanks.
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Thanks for updates ;)