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NGS tools for the novice

Handy tools for processing of next generation sequencing (NGS) data

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Next generation sequencing methods produce huge amounts of sequence data that require fast and robust automated bioinformatic solutions for initial processing and analysis.

This collection of simple Perl scripts is adressed to scientists doing research that bases on high throughput genomic/transcriptomic data. It does not require any bioinformatic expertise.
The scripts perform fundamental processing steps like sorting sequences by TAGs, FASTQ to FASTA conversion, filtering and counting of redundant sequences, individually adjustable FASTQ quality filtering or basic analyses like base count and analysis of sequence length distribution.

Therefore, the NGS toolbox provides a wide repertoire of possibilities without presuming a bioinformatic expertise of the user.

The NGS toolbox is planed to be subject to permanent extension.

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