MspJI-seq_pipeline1.0

beta

an pipeline for MspJI-digesting sequencing in DNA methylation study

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BSD Linux

Description

MspJI-seq_pipeline1.0 is pipeline for MspJI-digesting sequencing in DNA methylation study. Modification-dependent restriction endonuclease MspJI digestion coupled with next generation sequencing could estimate the methylation state of "CNNR" Cytosine location in the genome by mapping high throughput reads to the reference sequences. MspJI-seq_pipeline1.0 is designed to be a general-purpose mapping program to handle these special characteristics of MspJI-seq. Its alignment is based on the open source program SOAP (Short Oligo Alignment Program), and mCNNR sites recognition is performed by regular expression in perl.

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Additional Project Details

Languages

English

Intended Audience

Science/Research

Programming Language

Perl

Registered

2013-04-23
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