The Multi-purpose Automated Genome Project Investigation Environment (MAGPIE, http://magpie.ucalgary.ca/), is a software package for the automated curation and presentation of DNA and protein sequences. Freely available under terms of Apache license v2.0
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I have used one version or another of this project for > 10 years. As long as you are comfortable with the Unix filesystem, it's well worth the effort. Beware of disk space for databank searching and for output files!
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IntEnz is the name for the Integrated relational Enzyme database. IntEnz contains data curated and approved by the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology (NC-IUBMB).
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