PCR primers / probes design from multiple & degenerate sequences

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Gemi is fast, automated and user-friendly tool to design primers and probes for polymerase chain reaction (PCR).
Gemi accepts multiple aligned and long DNA and RNA sequences with degenerate nucleotide (non-A/C/G/T bases).
It can be used for quantitative/real-time PCR, conventional and Sanger sequencing (qPCR, rt-PCR, etc.).

* Note 1: In version 1.2, Td is calculated, but in version 1.3, the Tm and salt adjustment are considered.
* Note 2: If no oligo was reported, The search options/parameters should be changed as described in manual.

Article: PMID: 23316117, PMCID: PMC3535827

Source codes (C#, C++ and Python codes) will be available upon request.

Gemi Web Site


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Additional Project Details

Intended Audience

Government, Science/Research, Education, Agriculture

User Interface

.NET/Mono, Win32 (MS Windows)

Programming Language



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