PCR primers / probes design from multiple & degenerate sequences

Add a Review
12 Downloads (This Week)
Last Update:
Download Gemi v1.5
Browse All Files
BSD Windows Mac Linux



Gemi is fast, automated and user-friendly tool to design primers and probes for polymerase chain reaction (PCR).
Gemi accepts multiple aligned and long DNA and RNA sequences with degenerate nucleotide (non-A/C/G/T bases).
It can be used for quantitative/real-time PCR, conventional and Sanger sequencing (qPCR, rt-PCR, etc.).

* Notes
1. The latest version is 1.5.
2. In version 1.2, Td is calculated, but in version 1.3, the Tm and salt adjustment are considered.
3. If no oligo was reported, The search options/parameters should be changed as described in manual.

For details, please refer to the README file and user's guide, or contact the author.

Article: PMID: 23316117, PMCID: PMC3535827

Source codes (C#, C++ and Python codes) will be available upon request.

Gemi Web Site


Write a Review

User Reviews

Be the first to post a review of Gemi!

Additional Project Details

Intended Audience

Government, Science/Research, Education, Agriculture

User Interface

.NET/Mono, Win32 (MS Windows)

Programming Language



Screenshots can attract more users to your project.
Features can attract more users to your project.