PCR primers / probes design from multiple & degenerate sequences

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Gemi is fast, automated and user-friendly tool to design polymerase chain reaction (PCR) primers.
Gemi accepts multiple aligned and long DNA and RNA sequences with degenerate nucleotide (non-A/C/G/T bases).
It can be used for quantitative/real-time PCR, conventional and Sanger sequencing (qPCR, rtPCR). Gemi accepts
The programs are as the following:
1. The first program is to design PCR primers from multiple sequence alignment.
2. Program to convert ClustalW format (.aln), Phylip (.phy) and (.gde) formats to Fasta format.
3. Reverse and/or complement program is to find the reverse and complement counterpart of single or multiple sequences.

Please cite to Sobhy, H, Colson, P; Gemi: PCR Primers Prediction from Multiple Alignments, Comparative and Functional Genomics 2012; doi:10.1155/2012/783138.

Source codes (C#, C++ and Python codes) will be available upon request.

Gemi Web Site

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Additional Project Details

Intended Audience

Government, Science/Research, Education, Agriculture

User Interface

.NET/Mono, Win32 (MS Windows)

Programming Language




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