Gemi is fast, automated and user-friendly tool to design primers and probes for polymerase chain reaction (PCR).
Gemi accepts multiple aligned and long DNA and RNA sequences with degenerate nucleotide (non-A/C/G/T bases).
It can be used for quantitative/real-time PCR, conventional and Sanger sequencing (qPCR, rt-PCR, etc.).
1. The latest version is 1.5.
2. In version 1.2, Td is calculated, but in version 1.3, the Tm and salt adjustment are considered.
3. If no oligo was reported, The search options/parameters should be changed as described in manual.
For details, please refer to the README file and user's guide, or contact the author.
Article: PMID: 23316117, PMCID: PMC3535827
Source codes (C#, C++ and Python codes) will be available upon request.
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