GATA

I am running a PC with Windows Vista


I have installed the standalone blast 2 sequences from NCBI (version blast-2.2.18-ai32-win32) I have this working independently so I can enter two related sequences and perform a Blast search.

I gave the following inputs into GATA

#Single Fasta reference sequence
C:\BGsequences\The8.5GeneFASTA.txt

#Multi Fasta comparitive Sequences
C:\BGsequences\Reverse_complemented_CTBG_from_ensamble_080909.txt

#bl2seq blast program
C:\Blast\bin\bl2seq.exe

#Folder for saving
C:\BGsequences

#base name
ctbg

I then receive the error

"humm nothing came back from the bl2seq program! The problem may be due to a lack of homology between your test sequences try using the demo sequences Alternatively there may be something wrong with the bl2seq blast executable try a diferent version or download a new copy from ..."

The Help button also dosn't work

I get the error "Problem! Are you off line? java.io.FileNotFoundException: .\documents\AlignerHelp.html (The system cannot find the path specified)"


Can anyone help?