ASpipe is a pipeline to process GeneSeqer/GMAP alignments and identify alternative splicing (AS) events from the alignments. It requires unix bash, perl 5.0+ with DBI module and MySQL5.0+ to run properly.
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Version 2.1.5 Updated: Oct 17, 2008 Add "commit" to some insert syntax. As discovered on some machine, the insertion was done in buffer, another process started before the autocommit, which will cause wrong eTranscript prediction. Version 1.9 Updated: May 21 2008 (1) library/AS.pl: Fixed a bug in AS.pl where some IntronR events was predicted wrong (2) library/MergeExon.pl Consider fuzzy border option, some terminal exons may have splicing site not predicted well (3) library/cluster.pl Restructure the algorithm of clustering EST alignments, merge single exon with its neighboring clusters. (4) library/GetDBSeq.pl, bin/ASpipe.pl Add a new 'Hybrid' method of retrieving genome sequences, requires fastacmd and formated genomic database. Reduced memory usage. (5) bin/ASIPtoGFF3.pl Assign ? as the orientation to single exon Version 1.8 Updated: May 15 2008 (1) Fixed known bugs as of May 15 2008 (2) Cross-species EST model incoporated (3) Read input from GFF format file (4) Output in GFF format file
A stable version of ASpipe (ASpipe2.1.5) is now available at SourceForge. The new version fixed previous minor bugs and added some new features including output in GFF format. An perl-cgi based browser (ASviewer) to visualize the ASpipe data is available upon request. Please cite the following paper if you are using ASpipe: (1) Wang, B.-B., O'Toole, M., Brendel, V. & Young, N.D (2008) BMC Plant Biology Cross-Species EST Alignments Reveal Novel and Conserved Alternative Splicing Events in Legumes (2) Wang, B.-B. and Brendel, V. (2006) PNAS Genome-wide comparative analysis of alternative splicing in plants
Version 1.0.1 1/26/2007 (1) Updated ASpipe.pl, now write some important parameters to the log. (2) Updated GMAP_extract.pl, Fixed GMAP orientation confusion problem by reverse the genome strand when cDNA is antisense. See details in the script.
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