#133 correctPacBio in 550Mb genome

Assembly_analysis
open
nobody
None
5
2012-01-26
2012-01-26
BioTech
No

I have assembled 100X illumina reads and 1X Pacbio reads with CA7.0 and the genome size is approximately 550Mb.

To assemble illumina reads with 1X Pacbio RS data, I performed correctPacBio with 20X illumina reads.
However, 95% in length (Pacbio reads) were discarded after correctPacBio.

40Mb in length -> 2Mb in length

How to improve the efficiency of correctPacBio?
Shoud I reduce the value of merSize and ovlMerLen?
or Shoud I perform correctPacBio with more illumina reads?

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