I am new to the gap4 package. I am having problem with pregap4 (the tutorial "Mutation detection using the staden package"). I got the following complaint:
SEQ HS14680: Warning, TN command returned empty string
SEQ HS14680: Warning, PR command returned empty string
and a lot of mutant/polymorphic nucleotide positions were not highlighted in the subsequent gap4 walk-through.
Anybody know if how to fix the warning (I try to download a fresh copy of the HS14680.embl, etc; but none of them helped.) and if the two issues might be related.
Thanks a lot,