Trev: problem when viewing 454 sff files

2007-07-05
2013-04-18
  • Hi

    I have two sets of experiments, one from a international company and one done locally.

    When I view the sff flowgrams of the international data in trev, I get a lot of discrepancies of basecall vs. peak color (see http://www.bi.up.ac.za/~fourie/flowgram.jpg\).

    When i view the locally-generated data, everything is perfect...

    Has anyone else experienced something like this?

    Any info would be appreciated.

    Kindest regards!

    Fourie

    --------------
    Prof Fourie Joubert
    Associate Professor
    Bioinformatics and Computational Biology Unit
    Department of Biochemistry
    University of Pretoria
    fjoubert@postino.up.ac.za
    http://www.bi.up.ac.za
    Tel. +27-12-420-5802
    Fax. +27-12-420-5800