I have two sets of experiments, one from a international company and one done locally.
When I view the sff flowgrams of the international data in trev, I get a lot of discrepancies of basecall vs. peak color (see http://www.bi.up.ac.za/~fourie/flowgram.jpg\).
When i view the locally-generated data, everything is perfect...
Has anyone else experienced something like this?
Any info would be appreciated.
Prof Fourie Joubert
Bioinformatics and Computational Biology Unit
Department of Biochemistry
University of Pretoria