On Wed, Mar 9, 2011 at 3:50 PM, kanika sharma <ksharma997@gmail.com> wrote:


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I hope this makes my point clear

1BX7...the author determined structure is monomeric..PISA generate dimeric assembly

REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000   

Remark 350 in orthogonal coordinates

REMARK 350
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC
REMARK 350 SOFTWARE USED: PISA 1.18 
REMARK 350 TOTAL BURIED SURFACE AREA FOR THE COMPLEX:       2330 A**2
REMARK 350 SURFACE AREA FOR THE COMPLEX:                    6730 A**2
REMARK 350 GAIN IN SOLVENT FREE ENERGY:                      -49 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350   BIOMT1   2  0.000000 -1.000000  0.000000       75.42600
REMARK 350   BIOMT2   2 -1.000000  0.000000  0.000000       75.42600
REMARK 350   BIOMT3   2  0.000000  0.000000 -1.000000      -33.90600


can some one tell how it is???is it that in crystal study no dimeric form is seen???

and what could be the code for this BIOMT transformations...



Regards,
Kanika
create 1bx7_a, 1bx7
    alter_state 1, 1bx7_a,(x,y,z)=(what should be written here)