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#22 Probes (internal oligos) can be designed in excluded regions

open
nobody
5
2013-02-05
2007-12-07
richard
No

Internal oligos in Primer3 don't respect the excluded region option. To reproduce, just run a design with "pick hybridization probe" then go back and add an excluded region to somewhere in the binding region of the probe and run design again.

Restult:

1 TAGGGTTGTAAAACTCTTTCGCCGGTTAGGATAATGACGTTAACCGGAGAAGAAGCCCCG
>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^XXXXXXXXXX^^^^^

61 GCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGGGCTAGCGT
<<<<<<<<<<<<<<<<<<<

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