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#15 Probe design 2

open
nobody
None
3
2013-02-05
2006-12-05
Untergasser
No

It would be good if you could request that one of the primers goes over a certain nucleotide (great for RT-PCRs when you want to span a intron/exon boundary).

It would be great if there would be more flexibility in the primer included/target/excluded regions. It would be good if they could be selected independently in combination with the boundary tool.

Discussion

  • Steve Rozen
    Steve Rozen
    2006-12-06

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    I complete agree.

    I suggest we try to get some more molecular biologists and involved, and
    start a serious (and time-limited) requirements assessment / prioritiazation
    discussion.

     
  • Charles Plessy
    Charles Plessy
    2006-12-28

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    For designing primers over intron/exon junctions, I have cheated primer3 by indicating the splice junction (-10/+10) as a hybridisation oligo and relaxing the corresponding parameters (since the splice junction is not necessarly a good hybridisation oligo). The primers have not yet been tested in real life, but their design seems OK.

     
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    gaattc