#13 Cloning Primers

open
nobody
3
2013-02-05
2006-12-05
Untergasser
No

For cloning it is often required to add sequences like restriction sites or recombination sites 5' to the primer sequence.

It would be good if two sequences, one for forward and one for reverse could be given to primer3 and primer3 would add it to all its primers before it does its calculations to find the best pairs. Like that it can check them for binding and primer dimers etc.

Discussion

  • Steve Rozen
    Steve Rozen
    2006-12-06

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    As per e-mail discussion, I agree.