#12 checking primers

open
nobody
3
2013-02-05
2006-12-05
Untergasser
No

Bug: If primer3 is used to validate primers by putting them in primer_left_input and a given sequence it does not do it if the primer is not in the size limits. Also if the primer_pick_anyway is 1.

It would be good if we would have a way to analyse a primer pair with and without a sequence and to get all calculatable parameters (TM GC SELF ANY etc).

It would be also great if it could handle a list of primers for forward and for reverse and return the best pair.

Discussion

  • Steve Rozen
    Steve Rozen
    2006-12-06

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    I agree on all these