#13 Intron size for RT-PCR

open
nobody
None
5
2008-10-08
2008-10-08
Anonymous
No

Hi,
Thanks for a good program! I find it the best solution for designing Q-PCR primers. However it is a bit annoying that you have to check every primer pair manually to confirm that the intron they span is big enough. If you ever make any improvements in the program, one showing the intron sizes or an option to set minimum intron size would be greatly appreciated!

Best, Maria Frank
Karolinska institutet

Discussion

  • Owen Marshall
    Owen Marshall
    2008-10-08

    Yes, I've been thinking about this too. Of course, the "View intron/exon structure" button gives you a fair idea, but I agree, it's sub-optimal. I like the minimum intron size option idea.

    Thanks for the feedback,
    Owen