first i want to say i am an absolute beginner, so i am doing microarray analysis for the first time.
I did microarray analysis with cells, as common reference i selected tissue. I used Genepix to visualize the arrays - in some spots the background signal is higher than the sample median signal…so in the end there is an error concerning the log ratio, because youn can not compute a negative log.
When i put the data into MeV, these error log2´s are named "NaN". These "error genes" will not be included in my analysis, am i right? But what can i do to include these data? I hope somebody can help me, i would be happy!