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qPCR

Ben
2009-12-10
2013-05-20
  • Ben
    Ben
    2009-12-10

    Hi,

    Is it possible to load and analyse normalized qPCR data in MeV in order to make clustering, classification and maybe statistical analysis ?
    If not, is this could be interresting as an implementation of MeV ?

    Many thanks,

    Ben

     
  • Eleanor Howe
    Eleanor Howe
    2009-12-10

    Hi there Ben,

    You should be able to get qPCR data into MeV.
    MeV can load and analyze pretty much any data that is a matrix of floating-point numbers. The MeV manual has some instructions about the file formats that MeV can load from. I'd suggest the TDMS (tab-delimited multiple sample) file format as your easiest option.

    Eleanor

     
  • Ben
    Ben
    2009-12-11

    Hi Eleanor,

    Thanks for your answer and advice.

    I will try this as soon as possible but I have an other question about the data to load : is it better to have log transformed data or it doesn't mater ?
    Because I usually use MeV with normalized microarray data that are log transformed.
    Is the approach with qPCR should be the same ?

    Thanks.

    Ben

     
  • Eleanor Howe
    Eleanor Howe
    2009-12-11

    Hi Ben,

    It doesn't really matter if your data is log transformed or not. MeV will treat it the same way, regardless.

    Eleanor

     
  • Ben
    Ben
    2009-12-11

    thanks a lot.

    Regards

    Ben