#1129 Adjust automatic annotations for P30958

UniProt
open
rach_huntley
5
2014-02-19
2013-12-09
RFoulger
No

Hi,

Please adjust the automatic interpro2go and hamap2go annotation for P30958 Mfd_ecoli: It is NOT GO:0004386 helicase activity (see Uniprot entry and refs in pmid:22331906)

Thanks,
Birgit

(transferred from the GO term request SF tracker:
https://sourceforge.net/p/geneontology/ontology-requests/10566/)

Discussion

  • rach_huntley
    rach_huntley
    2013-12-09

    Hi Birgit,

    I notice that this entry has a manual annotation to GO:0008026 ATP-dependent helicase activity from PMID:8465200. Do you think this is an incorrect annotation?

    Thanks,
    Rachael.

     
  • rach_huntley
    rach_huntley
    2014-02-18

    Hi Birgit,

    Do you have any follow up to this?

    Thanks,
    Rachael.

     
  • Birgit Meldal
    Birgit Meldal
    2014-02-18

    Hi Rachael,

    Sorry, I didn't get an email notification on this ticket as you created it :( I just saw it in the Jenkins update...

    I think the manual annotation from PMID:8465200 refers to the 'helicase motifs' but no helicase activity has so far been demonstrated, hence the quote from UniProt: 'Has ATPase activity, which is required for removal of stalled RNAP, but seems to lack helicase activity.'

    Does that make sense?

    Regards,
    Birgit

     
  • rach_huntley
    rach_huntley
    2014-02-18

    Hi Birgit,

    I have created a NOT annotation to helicase activity using PMID:7876262. This combination of protein accession and GO term will now go onto the annotation blacklist, which will have the effect of preventing electronic annotations from being created. You should see the IEAs disappear from this record next week.

    Thanks,
    Rachael.

     
  • Birgit Meldal
    Birgit Meldal
    2014-02-18

    Perfect!

    Thanks,
    Birgit

     
  • Hi,

    I had a look at the InterPro entries responsible for the wrong IEA annotation (IPR005118, IPR004576 and IPR011545). They should not have had the term 'helicase activity' in the first place, as these proteins have 'helicase domains' but no helicase activity (they are translocases) as Birgit was mentioning. So the helicase annotation from these entries is gone now! (won't be visible till our next release).

    Cheers,
    Amaia

     
  • Birgit Meldal
    Birgit Meldal
    2014-02-19

    Thanks, Amaia!