Can you please provide more information about your calculation; e.g., including the APBS input file and what you mean by a “high” energy?
Pacific Northwest National Laboratory
From: Arnab Nayek [mailto:firstname.lastname@example.org]
Sent: Tuesday, August 30, 2011 9:38 PM
Subject: [Apbs-users] total electrostatic energy of a native protein can be positive
I am interested to do some APBS calculation in my research work. Actually I want to calculate the salt bridge energy of a protein. So for that purpose I follow a model and run the APBS for electrostatic energy calculation. APBS is running well but the energy which it produce is thought to be very high and also the problem is the total electrostatic energy of a native protein, which APBS produce is always positive value. Is it true? I have a confusion about this. Please help me to correct my problem.
PhD scholar (Bioinformatics)
Dept. Of Biotechnology
Burdwan University, WB, India